Characterization of Phytophthora hybrids from ITS clade 6 associated with riparian ecosystems in South Africa and Australia.
Identifieur interne : 001363 ( Main/Exploration ); précédent : 001362; suivant : 001364Characterization of Phytophthora hybrids from ITS clade 6 associated with riparian ecosystems in South Africa and Australia.
Auteurs : Jan H. Nagel [Afrique du Sud] ; Marieka Gryzenhout ; Bernard Slippers ; Michael J. Wingfield ; Giles E St J. Hardy ; Michael J C. Stukely ; Treena I. BurgessSource :
- Fungal biology [ 1878-6146 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN intergénique (génétique), ADN mitochondrial (génétique), Australie (MeSH), Données de séquences moléculaires (MeSH), Phylogenèse (MeSH), Phytophthora (classification), Phytophthora (croissance et développement), Phytophthora (génétique), Phytophthora (isolement et purification), République d'Afrique du Sud (MeSH), Écosystème (MeSH).
- MESH :
- croissance et développement : Phytophthora.
- génétique : ADN intergénique, ADN mitochondrial, Phytophthora.
- isolement et purification : Phytophthora.
- Australie, Données de séquences moléculaires, Phylogenèse, République d'Afrique du Sud, Écosystème.
- Wicri :
- geographic : Australie, Afrique du Sud.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Intergenic, DNA, Mitochondrial.
- geographic : Australia, South Africa.
- classification : Phytophthora.
- genetics : Phytophthora.
- growth & development : Phytophthora.
- isolation & purification : Phytophthora.
- Ecosystem, Molecular Sequence Data, Phylogeny.
Abstract
Surveys of Australian and South African rivers revealed numerous Phytophthora isolates residing in clade 6 of the genus, with internal transcribed spacer (ITS) gene regions that were either highly polymorphic or unsequenceable. These isolates were suspected to be hybrids. Three nuclear loci, the ITS region, two single copy loci (antisilencing factor (ASF) and G protein alpha subunit (GPA)), and one mitochondrial locus (cytochrome oxidase c subunit I (coxI)) were amplified and sequenced to test this hypothesis. Abundant recombination within the ITS region was observed. This, combined with phylogenetic comparisons of the other three loci, confirmed the presence of four different hybrid types involving the three described parent species Phytophthora amnicola, Phytophthora thermophila, and Phytophthora taxon PgChlamydo. In all cases, only a single coxI allele was detected, suggesting that hybrids arose from sexual recombination. All the hybrid isolates were sterile in culture and all their physiological traits tended to resemble those of the maternal parents. Nothing is known regarding their host range or pathogenicity. Nonetheless, as several isolates from Western Australia were obtained from the rhizosphere soil of dying plants, they should be regarded as potential threats to plant health. The frequent occurrence of the hybrids and their parent species in Australia strongly suggests an Australian origin and a subsequent introduction into South Africa.
DOI: 10.1016/j.funbio.2013.03.004
PubMed: 23719220
Affiliations:
Links toward previous steps (curation, corpus...)
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Nagel</name><affiliation wicri:level="1"><nlm:affiliation>Department of Genetics, Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa. jan.nagel@fabi.up.ac.za</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Genetics, Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002</wicri:regionArea><wicri:noRegion>Pretoria 0002</wicri:noRegion></affiliation></author><author><name sortKey="Gryzenhout, Marieka" sort="Gryzenhout, Marieka" uniqKey="Gryzenhout M" first="Marieka" last="Gryzenhout">Marieka Gryzenhout</name></author><author><name sortKey="Slippers, Bernard" sort="Slippers, Bernard" uniqKey="Slippers B" first="Bernard" last="Slippers">Bernard Slippers</name></author><author><name sortKey="Wingfield, Michael J" sort="Wingfield, Michael J" uniqKey="Wingfield M" first="Michael J" last="Wingfield">Michael J. Wingfield</name></author><author><name sortKey="Hardy, Giles E St J" sort="Hardy, Giles E St J" uniqKey="Hardy G" first="Giles E St J" last="Hardy">Giles E St J. Hardy</name></author><author><name sortKey="Stukely, Michael J C" sort="Stukely, Michael J C" uniqKey="Stukely M" first="Michael J C" last="Stukely">Michael J C. Stukely</name></author><author><name sortKey="Burgess, Treena I" sort="Burgess, Treena I" uniqKey="Burgess T" first="Treena I" last="Burgess">Treena I. Burgess</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23719220</idno><idno type="pmid">23719220</idno><idno type="doi">10.1016/j.funbio.2013.03.004</idno><idno type="wicri:Area/Main/Corpus">001273</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001273</idno><idno type="wicri:Area/Main/Curation">001273</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001273</idno><idno type="wicri:Area/Main/Exploration">001273</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization of Phytophthora hybrids from ITS clade 6 associated with riparian ecosystems in South Africa and Australia.</title><author><name sortKey="Nagel, Jan H" sort="Nagel, Jan H" uniqKey="Nagel J" first="Jan H" last="Nagel">Jan H. Nagel</name><affiliation wicri:level="1"><nlm:affiliation>Department of Genetics, Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa. jan.nagel@fabi.up.ac.za</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Genetics, Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002</wicri:regionArea><wicri:noRegion>Pretoria 0002</wicri:noRegion></affiliation></author><author><name sortKey="Gryzenhout, Marieka" sort="Gryzenhout, Marieka" uniqKey="Gryzenhout M" first="Marieka" last="Gryzenhout">Marieka Gryzenhout</name></author><author><name sortKey="Slippers, Bernard" sort="Slippers, Bernard" uniqKey="Slippers B" first="Bernard" last="Slippers">Bernard Slippers</name></author><author><name sortKey="Wingfield, Michael J" sort="Wingfield, Michael J" uniqKey="Wingfield M" first="Michael J" last="Wingfield">Michael J. Wingfield</name></author><author><name sortKey="Hardy, Giles E St J" sort="Hardy, Giles E St J" uniqKey="Hardy G" first="Giles E St J" last="Hardy">Giles E St J. Hardy</name></author><author><name sortKey="Stukely, Michael J C" sort="Stukely, Michael J C" uniqKey="Stukely M" first="Michael J C" last="Stukely">Michael J C. Stukely</name></author><author><name sortKey="Burgess, Treena I" sort="Burgess, Treena I" uniqKey="Burgess T" first="Treena I" last="Burgess">Treena I. Burgess</name></author></analytic><series><title level="j">Fungal biology</title><idno type="ISSN">1878-6146</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Australia (MeSH)</term><term>DNA, Intergenic (genetics)</term><term>DNA, Mitochondrial (genetics)</term><term>Ecosystem (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phytophthora (classification)</term><term>Phytophthora (genetics)</term><term>Phytophthora (growth & development)</term><term>Phytophthora (isolation & purification)</term><term>South Africa (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN intergénique (génétique)</term><term>ADN mitochondrial (génétique)</term><term>Australie (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Phytophthora (classification)</term><term>Phytophthora (croissance et développement)</term><term>Phytophthora (génétique)</term><term>Phytophthora (isolement et purification)</term><term>République d'Afrique du Sud (MeSH)</term><term>Écosystème (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Intergenic</term><term>DNA, Mitochondrial</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Australia</term><term>South Africa</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN intergénique</term><term>ADN mitochondrial</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Ecosystem</term><term>Molecular Sequence Data</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Australie</term><term>Données de séquences moléculaires</term><term>Phylogenèse</term><term>République d'Afrique du Sud</term><term>Écosystème</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Australie</term><term>Afrique du Sud</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Surveys of Australian and South African rivers revealed numerous Phytophthora isolates residing in clade 6 of the genus, with internal transcribed spacer (ITS) gene regions that were either highly polymorphic or unsequenceable. These isolates were suspected to be hybrids. Three nuclear loci, the ITS region, two single copy loci (antisilencing factor (ASF) and G protein alpha subunit (GPA)), and one mitochondrial locus (cytochrome oxidase c subunit I (coxI)) were amplified and sequenced to test this hypothesis. Abundant recombination within the ITS region was observed. This, combined with phylogenetic comparisons of the other three loci, confirmed the presence of four different hybrid types involving the three described parent species Phytophthora amnicola, Phytophthora thermophila, and Phytophthora taxon PgChlamydo. In all cases, only a single coxI allele was detected, suggesting that hybrids arose from sexual recombination. All the hybrid isolates were sterile in culture and all their physiological traits tended to resemble those of the maternal parents. Nothing is known regarding their host range or pathogenicity. Nonetheless, as several isolates from Western Australia were obtained from the rhizosphere soil of dying plants, they should be regarded as potential threats to plant health. The frequent occurrence of the hybrids and their parent species in Australia strongly suggests an Australian origin and a subsequent introduction into South Africa.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23719220</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>25</Day></DateCompleted><DateRevised><Year>2013</Year><Month>05</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1878-6146</ISSN><JournalIssue CitedMedium="Internet"><Volume>117</Volume><Issue>5</Issue><PubDate><Year>2013</Year><Month>May</Month></PubDate></JournalIssue><Title>Fungal biology</Title><ISOAbbreviation>Fungal Biol</ISOAbbreviation></Journal><ArticleTitle>Characterization of Phytophthora hybrids from ITS clade 6 associated with riparian ecosystems in South Africa and Australia.</ArticleTitle><Pagination><MedlinePgn>329-47</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.funbio.2013.03.004</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1878-6146(13)00049-4</ELocationID><Abstract><AbstractText>Surveys of Australian and South African rivers revealed numerous Phytophthora isolates residing in clade 6 of the genus, with internal transcribed spacer (ITS) gene regions that were either highly polymorphic or unsequenceable. These isolates were suspected to be hybrids. Three nuclear loci, the ITS region, two single copy loci (antisilencing factor (ASF) and G protein alpha subunit (GPA)), and one mitochondrial locus (cytochrome oxidase c subunit I (coxI)) were amplified and sequenced to test this hypothesis. Abundant recombination within the ITS region was observed. This, combined with phylogenetic comparisons of the other three loci, confirmed the presence of four different hybrid types involving the three described parent species Phytophthora amnicola, Phytophthora thermophila, and Phytophthora taxon PgChlamydo. In all cases, only a single coxI allele was detected, suggesting that hybrids arose from sexual recombination. All the hybrid isolates were sterile in culture and all their physiological traits tended to resemble those of the maternal parents. Nothing is known regarding their host range or pathogenicity. Nonetheless, as several isolates from Western Australia were obtained from the rhizosphere soil of dying plants, they should be regarded as potential threats to plant health. The frequent occurrence of the hybrids and their parent species in Australia strongly suggests an Australian origin and a subsequent introduction into South Africa.</AbstractText><CopyrightInformation>Copyright © 2013 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nagel</LastName><ForeName>Jan H</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Department of Genetics, Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa. jan.nagel@fabi.up.ac.za</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gryzenhout</LastName><ForeName>Marieka</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Slippers</LastName><ForeName>Bernard</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Wingfield</LastName><ForeName>Michael J</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Hardy</LastName><ForeName>Giles E St J</ForeName><Initials>GE</Initials></Author><Author ValidYN="Y"><LastName>Stukely</LastName><ForeName>Michael J C</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Burgess</LastName><ForeName>Treena I</ForeName><Initials>TI</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>03</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Fungal Biol</MedlineTA><NlmUniqueID>101524465</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021901">DNA, Intergenic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004272">DNA, Mitochondrial</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001315" MajorTopicYN="N" Type="Geographic">Australia</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021901" MajorTopicYN="N">DNA, Intergenic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004272" MajorTopicYN="N">DNA, Mitochondrial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017753" MajorTopicYN="N">Ecosystem</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013019" MajorTopicYN="N" Type="Geographic">South Africa</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>09</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>03</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>03</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>5</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>5</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23719220</ArticleId><ArticleId IdType="pii">S1878-6146(13)00049-4</ArticleId><ArticleId IdType="doi">10.1016/j.funbio.2013.03.004</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Afrique du Sud</li></country></list><tree><noCountry><name sortKey="Burgess, Treena I" sort="Burgess, Treena I" uniqKey="Burgess T" first="Treena I" last="Burgess">Treena I. Burgess</name><name sortKey="Gryzenhout, Marieka" sort="Gryzenhout, Marieka" uniqKey="Gryzenhout M" first="Marieka" last="Gryzenhout">Marieka Gryzenhout</name><name sortKey="Hardy, Giles E St J" sort="Hardy, Giles E St J" uniqKey="Hardy G" first="Giles E St J" last="Hardy">Giles E St J. Hardy</name><name sortKey="Slippers, Bernard" sort="Slippers, Bernard" uniqKey="Slippers B" first="Bernard" last="Slippers">Bernard Slippers</name><name sortKey="Stukely, Michael J C" sort="Stukely, Michael J C" uniqKey="Stukely M" first="Michael J C" last="Stukely">Michael J C. Stukely</name><name sortKey="Wingfield, Michael J" sort="Wingfield, Michael J" uniqKey="Wingfield M" first="Michael J" last="Wingfield">Michael J. Wingfield</name></noCountry><country name="Afrique du Sud"><noRegion><name sortKey="Nagel, Jan H" sort="Nagel, Jan H" uniqKey="Nagel J" first="Jan H" last="Nagel">Jan H. Nagel</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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